Date of Award
Spring 6-5-2024
Degree Type
Thesis
Degree Name
MS Biology
Department
Biology
Advisor
Jessica Cottrell, PhD
Committee Member
Daniel Brian Nichols, PhD
Committee Member
Constantine Bitsaktsis, PhD
Keywords
manganese chloride, chondrogenesis, arachidonic acid, COX-2, PGE2
Abstract
Manganese is an essential trace element found in a broad range of foods and contributes to bone metabolism by supporting collagen synthesis, enhancing osteoblast function, and promoting bone development and growth. Chondrocytes contribute to this process by facilitating endochondral ossification, secreting extracellular matrix components, such as collagen and proteoglycans which serve as the foundation for osteoblasts to produce and mineralize bone tissue. However, in high doses MnCl2 has been shown to have a damaging effect on chondrocyte function. The objective of this study was to determine how increasing doses of MnCl2 impact chondrocytes and the activity of pro-inflammatory enzymes within the arachidonic acid pathway. ATDC5 chondrocytes were cultured with increasing doses of MnCl2 and assayed for the protein expression via immunoblotting, proteomics, and prostaglandin analysis. Our data shows that MnCl2 increases COX-2 expression, prostaglandin synthesis specifically PGE2, and inflammatory mediators. Elevated levels of PGE2 have been associated with cartilage degradation and osteoarthritis progression. This research may have implications for various physiological processes, including bone metabolism.
Recommended Citation
Schmittberger, Helena, "Characterization of COX-2 Induced Expression on Manganese Chloride Treated ATDC5 Chondrocytes" (2024). Seton Hall University Dissertations and Theses (ETDs). 3191.
https://scholarship.shu.edu/dissertations/3191
Signed Committee
