Date of Award
Fall 12-12-2022
Degree Type
Thesis
Degree Name
MS Biology
Department
Biology
Advisor
Daniel B. Nichols, PhD
Advisor
Suzanne Gantar, PhD
Committee Member
Constantine Bitsaktsis, PhD
Committee Member
Angela Klaus, PhD
Keywords
Glioblastoma Multiforme, T98G, Apoptosis, PPAR, Immunoblotting, SRB
Abstract
1,1-bis(3’idolyl)-1(aryl)methane compounds (BIM compounds) have been shown to have anti-cancer properties in colon cancer, bladder cancer, and leukemia cells. The purpose of this work was to determine if BIM compounds could be an effective treatment of glioblastoma multiforme. Sulforhodamine B (SRB) assays showed that 20µM of the BIM compounds could inhibit cellular proliferation of the T98G glioblastoma multiforme cell line over 72 hours. Then immunoblotting was used to analyze the molecular pathway induced by BIM compounds. An increase in the expression of both BAX and cleaved caspase 3 suggest BIM compounds activate programmed cell death, or apoptosis in glioblastoma cells. In conducting this work, I am hoping to contribute to the creation of a new treatment for glioblastoma multiforme that will minimize the negative side effects of traditional cancer treatments.
Recommended Citation
Brown, Margot C., "Bis-Indolyl Compounds and the Induction of Apoptosis in T98G Glioblastoma Multiforme Cells" (2022). Seton Hall University Dissertations and Theses (ETDs). 3046.
https://scholarship.shu.edu/dissertations/3046
Included in
Biochemistry Commons, Cancer Biology Commons, Cell Biology Commons
