Date of Award
Heping Zhou, Ph.D.
C.J. Urso, Ph.D.
D. Brian Nichols, Ph.D.
FATP, SLC27A, fatty acid, fatty acid uptake, fatty acid transport protein, N2a
The diverse composition of fats in the brain includes saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. Depending on the chain length, fatty acids may be short-chain, medium-chain, long-chain, and very long-chain fatty acids. For long-chain and very long-chain fatty acids, their uptake into the cells is usually mediated by fatty acid transporters. This study was designed to examine the role of fatty acid transport proteins (FATPs) in fatty acid uptake in Neuro-2a cells. While the mRNA expression levels of FATP1, FATP3, FATP5, and FATP6 were not significantly altered following 24 h treatment with different long chain fatty acids, the mRNA expression of FATP4 appeared to be upregulated following 24 h treatment with palmitic acid (PA). The uptake of long chain fatty acids was significantly lower in Neuro-2a cells with FATP1 or FATP3 knocked down, suggesting that they may play an important role in long chain fatty acid uptake in Neuro-2a cells.
Kim, Michelle, "Role of fatty acid transport proteins in fatty acid uptake in Neuro-2a cells" (2023). Seton Hall University Dissertations and Theses (ETDs). 3101.
Available for download on Saturday, May 21, 2033