Date of Award
Chemistry and Biochemistry
Nicholas H. Snow, Ph.D.
Wyatt R. Murphy, Ph.D.
Yuri V. Kazakevich, Ph.D.
Polyol induced extraction of drug molecules, Glucocorticoids, NSAIDs, UHPLC-MS/MS, LLE, PIE-UHPLC-MS/MS, LC-MS/MS
Polyol induced extraction (PIE) is an extraction technique developed and patented by Sowa Jr., Murphy, and Deshpande at Seton Hall University as a method of removal of water from acetonitrile. PIE is a technique that separates water from a mixture containing an organic liquid and water by adding a polyol to the mixture that leads to phase separation of aqueous and organic phase. The goal of this work was to extend the application of PIE and to demonstrate that PIE can be an alternative method for extraction of glucocorticoids and non-steroidal anti-inflammatory drugs (NSAIDS) which then can be analyzed using ultra high-performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-MS/MS). A multiple reaction monitoring transitions of selected precursor ion to selected product ions were optimized for each analyte for quantitative analysis. Optimization of liquid chromatography was carried out to determine chromatographic condition. For each application method validation was performed and percent recovery, linearity, limit of detection, limit of quantitation, and accuracy were determined for each compound.
Similarly, PIE was also applied for the extraction of NSAIDs from water into acetonitrile using glycerol as a phase separating agent. Residues of these drugs are also emerging pollutants in water. Eight NSAIDS were subjected to PIE and then analyzed using UHPLC-MS/MS. Additionally synthetic urine samples were spiked with NSAIDS and then were subjected to PIE and analyzed.
In this research work, PIE of glucocorticoids and their analysis using gas chromatography (GC) was performed to evaluate compatibility of PIE with gas chromatography. GC-TOFMS was used to carry out the analysis. Glucocorticoids were analyzed without any derivatization process.
Results obtained in this study showed that PIE has an amazing potential to be an extraction technique for drugs, especially glucocorticoids and NSAIDs. PIE is very simple, cost effective and user-friendly extraction technique that can be compatible with both liquid and gas chromatography.
Patel, Shipra, "Extending the application of Polyol-induced extraction for the analysis of Glucocorticoids and NSAIDs in water using UHPLC-MS/MS" (2022). Seton Hall University Dissertations and Theses (ETDs). 2985.