Date of Award


Degree Type


Degree Name

MS Biology




Allan Blake

Committee Member

Carolyn Bentivegna

Committee Member

Angela Klaus


Immigrant monocytes, Resident macrophages, Somatostatin (SRIF), RAW 264.7 cells


Immigrant monocytes and resident macrophages propagate the vertebrate innate immune response through cell migration and cytokine production. Monocytes responding to inflammatory challenge migrate into tissues, and as resident macrophages, release a major pro-inflammatory cytokine, tumor necrosis factor a (TNF-a). Macrophge cytokine synthesis is known to be under both negative and positive regulatory control. Recent studies have shown that somatostatin (SRIF) regulates monocyte and peritoneal macrophages. However, the effects of SRIF on RAW 264.7 cells, a transformed monocyte/macrophage cell line, have not been investigated. In the present study, SRIF effect on cytokine release in LPS stimulated RAW 264. 7 cells was examined. Somatostatin regulated monocyte response to LPS stimulation as reflected by decrease in TNF-a release. In particular, LPS showed temporal TNF-a release peaking at 2h. SRIF, 7 10 at physiological concentrations (10- M to 10- M) showed dose independent reduction 10 on TNF-a release. We found SRIF 10- M concentration inhibited TNF-a release to the 10 maximum at 2 h. Finally, we show SRIF 10- Mover a time course inhibited maximum TNF-a release at 2 h in LPS stimulated RAW 264.7 cells. Taken together, our results show that SRIF modulates TNF-a release in LPS stimulated RAW 264.7 cells.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.